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EOS, an Ikaros family zinc finger transcription factor, interacts with the HTLV-1 oncoprotein Tax and is downregulated in peripheral blood mononuclear cells of HTLV-1-infected individuals, irrespective of clinical statuses.

EOS, an Ikaros family zinc finger transcription factor, interacts with the HTLV-1 oncoprotein Tax and is downregulated in peripheral blood mononuclear cells of HTLV-1-infected individuals, irrespective of clinical statuses.
Posted by Seth

EOS plays an important role in maintaining the suppressive function of regulatory T cells (Treg), and a set of Tregs induced transformation into T helper-like cells, which are able to secrete proinflammatory cytokines in response to certain inflammatory signals. Meanwhile, a significant reduction in Treg activity along with the production of proinflammatory cytokines have been reported in patients with HTLV-1-associated myelopathy / tropical spastic paraparesis (HAM / TSP) .In this study, to test whether there is a change in the expression of EOS in mononuclear cells peripheral blood (PBMC) were from individuals HTLV-1-infected mainly HAM / TSP, we examined the expression of HTLV-1 genotype tax proviral load (PVL), and the mRNA expression of taxes, HBZ and EOS in HTLV- 1 infected individuals, including cell T adult leukemia / lymphoma (ATL), HAM / TSP, or asymptomatic carriers.

EOS expression levels of mRNA and protein in various HTLV-1-infected or uninfected human T cell line investigated.EOS also highly expressed at the protein level in most of HTLV-1 line-infected T cells, and added after the HTLV-1 Tax regulatory factors induced in the JPX-9 Tax-induced cell lines. Immunoprecipitation experiments showed physical interaction between EOS and Tax viral regulatory protein, but not HBZ. Meanwhile, there was a significant reduction in the level of mRNA in PBMCs EOS HTLV-1 infected individuals regardless of their clinical status.

We found an inverse correlation between the level of the EOS mRNA and HTLV-1 PVL in patients with ATL, and a positive correlation between the two load EOS mRNA and PVL, and EOS and load HBZ mRNA in patients with HAM / TSP, whereas this correlation was not observed in other clinical findings statuses .

These show that both Tax and HBZ can alter the expression of EOS through a mechanism that has not been determined, and dysregulation EOS expression in PBMCs HTLV-1 infected individuals may contribute to the pathological development of HTLV-1-related diseases, such as ATL and HAM / TSP.

 EOS, an Ikaros family zinc finger transcription factor, interacts with the HTLV-1 oncoprotein Tax and is downregulated in peripheral blood mononuclear cells of HTLV-1-infected individuals, irrespective of clinical statuses.
EOS, an Ikaros family zinc finger transcription factor, interacts with the HTLV-1 oncoprotein Tax and is downregulated in peripheral blood mononuclear cells of HTLV-1-infected individuals, irrespective of clinical statuses.

Downregulation of miR-4772-3p promote enhanced capacity of regulatory T cells in malignant pleural effusions by elevating the level of Helios.

malignant pleural effusion (MPE) is a complex condition of patients with advanced tumor. Further dissect immune cells infiltrate the microenvironment of malignant cells and are guaranteed to understand the immune-evasion mechanisms of tumor development and progression.

The possible involvement of microRNAs (miRNAs) in malignant pleural fluid were investigated using small RNA sequencing. regulatory T cells (Treg) markers (CD4, CD25, forkhead box P3) and Helios (also known as IKAROS Family Zinc Finger 2 [IKZF2]) were detected using flow cytometry. The expression level of miR-4772-3p IKZF2 and measured using real-time quantitative reverse transcription polymerase chain reaction. Interaction between miR-4772-3p and Helios determined using dual-luciferase reporter test.

Effects of miR-4772-3p on Helios expression was evaluated using in vitro system. test the correlation between miR-4772-3p and functional molecules Tregs that performed.Compared with non-malignant controls, patients with lung cancer non-small cell has an increased frequency of Tregs with MPE and Helios expression in peripheral blood mononuclear cells. the downregulation of miR-4772-3p verified inversely proportional to the frequency of Tregs Helios and Helios expression in the MPE. Overexpression of miR-4772-3p could inhibit the expression of Helios in vitro experiments.

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E02E0219-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat beta Endorphin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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Description: A sandwich ELISA for quantitative measurement of Rat beta Endorphin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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Description: A sandwich ELISA for quantitative measurement of Rat beta Endorphin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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E08E0219-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine beta Endorphin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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E08E0219-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine beta Endorphin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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E08E0219-96 1 plate of 96 wells
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Description: A sandwich ELISA for quantitative measurement of Canine beta Endorphin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig beta Endorphin ELISA kit

E07E0219-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Porcine beta Endorphin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig beta Endorphin ELISA kit

E07E0219-48 1 plate of 48 wells
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Description: A sandwich ELISA for quantitative measurement of Porcine beta Endorphin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig beta Endorphin ELISA kit

E07E0219-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Porcine beta Endorphin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Beta- Endorphin ELISA Kit

ELA-E0806r 96 Tests
EUR 1063.2

Cat beta Endorphin ELISA Kit

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Rat beta Endorphin ELISA Kit

MBS018689-10x96StripWells 10x96-Strip-Wells
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Rat beta Endorphin ELISA Kit

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Rat beta Endorphin ELISA Kit

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EUR 485

Rat beta Endorphin ELISA Kit

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EUR 3020

Rat beta Endorphin ELISA Kit

MBS732297-96StripWells 96-Strip-Wells
EUR 690

Goat beta Endorphin ELISA kit

E06E0219-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat beta Endorphin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat beta Endorphin ELISA kit

E06E0219-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Goat beta Endorphin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat beta Endorphin ELISA kit

E06E0219-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Goat beta Endorphin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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Human beta Endorphin ELISA Kit

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EUR 5685

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MBS720455-48StripWells 48-Strip-Wells
EUR 485

Human beta Endorphin ELISA Kit

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EUR 3020

Human beta Endorphin ELISA Kit

MBS720455-96StripWells 96-Strip-Wells
EUR 690

Mouse beta Endorphin ELISA kit

E03E0219-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Mouse beta Endorphin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse beta Endorphin ELISA kit

E03E0219-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Mouse beta Endorphin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse beta Endorphin ELISA kit

E03E0219-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Mouse beta Endorphin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human beta Endorphin ELISA kit

E01E0219-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human beta Endorphin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human beta Endorphin ELISA kit

E01E0219-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Human beta Endorphin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human beta Endorphin ELISA kit

E01E0219-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Human beta Endorphin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Camel beta Endorphin ELISA Kit

MBS096491-10x96StripWells 10x96-Strip-Wells
EUR 6725

Camel beta Endorphin ELISA Kit

MBS096491-48StripWells 48-Strip-Wells
EUR 550

Camel beta Endorphin ELISA Kit

MBS096491-5x96StripWells 5x96-Strip-Wells
EUR 3420

Camel beta Endorphin ELISA Kit

MBS096491-96StripWells 96-Strip-Wells
EUR 765

Mouse beta Endorphin ELISA Kit

MBS018158-10x96StripWells 10x96-Strip-Wells
EUR 6725

Mouse beta Endorphin ELISA Kit

MBS018158-48StripWells 48-Strip-Wells
EUR 550

Mouse beta Endorphin ELISA Kit

MBS018158-5x96StripWells 5x96-Strip-Wells
EUR 3420

Mouse beta Endorphin ELISA Kit

MBS018158-96StripWells 96-Strip-Wells
EUR 765

Human beta Endorphin ELISA Kit

MBS009924-10x96StripWells 10x96-Strip-Wells
EUR 6725

Human beta Endorphin ELISA Kit

MBS009924-48StripWells 48-Strip-Wells
EUR 550

Human beta Endorphin ELISA Kit

MBS009924-5x96StripWells 5x96-Strip-Wells
EUR 3420

Human beta Endorphin ELISA Kit

MBS009924-96StripWells 96-Strip-Wells
EUR 765

Goose beta Endorphin ELISA Kit

MBS011644-INQUIRE INQUIRE Ask for price

Sheep beta Endorphin ELISA Kit

MBS033331-10x96StripWells 10x96-Strip-Wells
EUR 6725

Sheep beta Endorphin ELISA Kit

MBS033331-48StripWells 48-Strip-Wells
EUR 550

Sheep beta Endorphin ELISA Kit

MBS033331-5x96StripWells 5x96-Strip-Wells
EUR 3420

Sheep beta Endorphin ELISA Kit

MBS033331-96StripWells 96-Strip-Wells
EUR 765

However, ectopic expression of Helios, induced Tregs reversed the effects caused by excessive mir-4772-3p. In addition, miR-4772-3p can regulate the expression of Helios by directly targeting IKZF2 mRNA.Downregulation of miR-4772-3p, by targeting Helios, contributing to the activities of Tregs increased in the microenvironment of MPE.

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An investigation of methylation pattern changes in the IKZF1 promoter in patients with childhood B-cell acute lymphoblastic leukemia.
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EOS, an Ikaros family zinc finger transcription factor, interacts with the HTLV-1 oncoprotein Tax and is downregulated in peripheral blood mononuclear cells of HTLV-1-infected individuals, irrespective of clinical statuses.

EOS, an Ikaros family zinc finger transcription factor, interacts with the HTLV-1 oncoprotein Tax and is downregulated in peripheral blood mononuclear cells of HTLV-1-infected individuals, irrespective of clinical statuses.

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