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EOS, an Ikaros family zinc finger transcription factor, interacts with the HTLV-1 oncoprotein Tax and is downregulated in peripheral blood mononuclear cells of HTLV-1-infected individuals, irrespective of clinical statuses.

EOS, an Ikaros family zinc finger transcription factor, interacts with the HTLV-1 oncoprotein Tax and is downregulated in peripheral blood mononuclear cells of HTLV-1-infected individuals, irrespective of clinical statuses.
Posted by Seth

EOS plays an important role in maintaining the suppressive function of regulatory T cells (Treg), and a set of Tregs induced transformation into T helper-like cells, which are able to secrete proinflammatory cytokines in response to certain inflammatory signals. Meanwhile, a significant reduction in Treg activity along with the production of proinflammatory cytokines have been reported in patients with HTLV-1-associated myelopathy / tropical spastic paraparesis (HAM / TSP) .In this study, to test whether there is a change in the expression of EOS in mononuclear cells peripheral blood (PBMC) were from individuals HTLV-1-infected mainly HAM / TSP, we examined the expression of HTLV-1 genotype tax proviral load (PVL), and the mRNA expression of taxes, HBZ and EOS in HTLV- 1 infected individuals, including cell T adult leukemia / lymphoma (ATL), HAM / TSP, or asymptomatic carriers.

EOS expression levels of mRNA and protein in various HTLV-1-infected or uninfected human T cell line investigated.EOS also highly expressed at the protein level in most of HTLV-1 line-infected T cells, and added after the HTLV-1 Tax regulatory factors induced in the JPX-9 Tax-induced cell lines. Immunoprecipitation experiments showed physical interaction between EOS and Tax viral regulatory protein, but not HBZ. Meanwhile, there was a significant reduction in the level of mRNA in PBMCs EOS HTLV-1 infected individuals regardless of their clinical status.

We found an inverse correlation between the level of the EOS mRNA and HTLV-1 PVL in patients with ATL, and a positive correlation between the two load EOS mRNA and PVL, and EOS and load HBZ mRNA in patients with HAM / TSP, whereas this correlation was not observed in other clinical findings statuses .

These show that both Tax and HBZ can alter the expression of EOS through a mechanism that has not been determined, and dysregulation EOS expression in PBMCs HTLV-1 infected individuals may contribute to the pathological development of HTLV-1-related diseases, such as ATL and HAM / TSP.

 EOS, an Ikaros family zinc finger transcription factor, interacts with the HTLV-1 oncoprotein Tax and is downregulated in peripheral blood mononuclear cells of HTLV-1-infected individuals, irrespective of clinical statuses.
EOS, an Ikaros family zinc finger transcription factor, interacts with the HTLV-1 oncoprotein Tax and is downregulated in peripheral blood mononuclear cells of HTLV-1-infected individuals, irrespective of clinical statuses.

Downregulation of miR-4772-3p promote enhanced capacity of regulatory T cells in malignant pleural effusions by elevating the level of Helios.

malignant pleural effusion (MPE) is a complex condition of patients with advanced tumor. Further dissect immune cells infiltrate the microenvironment of malignant cells and are guaranteed to understand the immune-evasion mechanisms of tumor development and progression.

The possible involvement of microRNAs (miRNAs) in malignant pleural fluid were investigated using small RNA sequencing. regulatory T cells (Treg) markers (CD4, CD25, forkhead box P3) and Helios (also known as IKAROS Family Zinc Finger 2 [IKZF2]) were detected using flow cytometry. The expression level of miR-4772-3p IKZF2 and measured using real-time quantitative reverse transcription polymerase chain reaction. Interaction between miR-4772-3p and Helios determined using dual-luciferase reporter test.

Effects of miR-4772-3p on Helios expression was evaluated using in vitro system. test the correlation between miR-4772-3p and functional molecules Tregs that performed.Compared with non-malignant controls, patients with lung cancer non-small cell has an increased frequency of Tregs with MPE and Helios expression in peripheral blood mononuclear cells. the downregulation of miR-4772-3p verified inversely proportional to the frequency of Tregs Helios and Helios expression in the MPE. Overexpression of miR-4772-3p could inhibit the expression of Helios in vitro experiments.

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Description: Recombinant Human Beta-Endorphin expressed in: Protein conjugation

OVA Conjugated Beta-Endorphin (bEP)

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  • EUR 226.34
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  • EUR 214.00
  • EUR 1284.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
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Description: Recombinant Mouse Beta-Endorphin expressed in: Protein conjugation

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  • EUR 1284.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Beta-Endorphin expressed in: Protein conjugation

Goat Beta- Endorphin ELISA Kit

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Goat beta Endorphin ELISA kit

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ELISA kit for Mouse Beta-Endorphin (Beta-EP)

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ELISA kit for Rat Beta-Endorphin (Beta-EP)

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Description: Quantitative sandwich ELISA for measuring Rat Beta-Endorphin (Beta-EP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

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Human beta Endorphin (bEP) ELISA Kit

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Mouse Beta-Endorphin (bEP) ELISA Kit

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Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

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Horse Beta-Endorphin (bEP) CLIA Kit

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Rat Beta-Endorphin (bEP) CLIA Kit

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Pig Beta-Endorphin (bEP) ELISA Kit

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Rabbit Beta-Endorphin (bEP) ELISA Kit

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Rat Beta-Endorphin (bEP) ELISA Kit

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Cow beta Endorphin (bEP) ELISA Kit

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Bovine Beta-Endorphin (bEP) ELISA Kit

DLR-bEP-b-48T 48T
EUR 547
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Beta-Endorphin (bEP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

However, ectopic expression of Helios, induced Tregs reversed the effects caused by excessive mir-4772-3p. In addition, miR-4772-3p can regulate the expression of Helios by directly targeting IKZF2 mRNA.Downregulation of miR-4772-3p, by targeting Helios, contributing to the activities of Tregs increased in the microenvironment of MPE.

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EOS, an Ikaros family zinc finger transcription factor, interacts with the HTLV-1 oncoprotein Tax and is downregulated in peripheral blood mononuclear cells of HTLV-1-infected individuals, irrespective of clinical statuses.

EOS, an Ikaros family zinc finger transcription factor, interacts with the HTLV-1 oncoprotein Tax and is downregulated in peripheral blood mononuclear cells of HTLV-1-infected individuals, irrespective of clinical statuses.

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